Will not start and alarm went off when I opened the door manually?

Hello,

It sounds like the anti theft was triggered, that's probably it does not want to start.
You will have to first reset the alarm system in order to start the vehicle.

Try this general reset procedure for Audi:

With the ignition key try to start the vehicle.
Exit the vehicle and lock all door with the windows up, then unlock the doors using the key fob the engine should start.
Disconnect the battery using the negative cable wait one minute and reconnect.

Here are some helpful information:

https://www.2carpros.com/articles/how-to-reset-a-security-system

Please let me know of any questions.

Thank you.