Okay, are you all set with how to test the AF sensor heater? If the heater is open circuit inside the sensor, the sensor will not function at all. These are nothing like conventional Oxygen sensors, the heaters in these get to extremely high temperatures for proper operation. They still send lean or rich exhaust data to the ECM, but they do it by current flow and not a voltage level. They are also very expensive. I recently purchased one for a 2007 Pathfinder I'm working on and the OEM was $307, but the customer could not afford that price, so I did find a Denso sensor on Rockauto com for around $100 which is an okay brand. So, if you find the sensor needs to be replaced check there.
Here is the link for your vehicle straight to the AF sensors, they call it an oxygen sensor here as well, but you're looking for the upstream sensor so Sensor 1 Bank 2.
https://www.rockauto.com/en/catalog/toyota,2006,highlander,3.3l+v6+electric/gas,1431836,exhaust+&+emission,oxygen+(o2)+sensor,5132
I see some Denso, Delphi and Bosch from about $105 to $185 range
The Bank 2 sensor is in the front of this vehicle, it's under the upper heat shield on the exhaust manifold. So, these ones are easier to get to, if you get the exhaust hot, they come out easier most of the time, just wear gloves. Here is the OEM part number as well.
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Tuesday, September 12th, 2023 AT 8:45 AM