Cylinder one misfire?

There are a number of components that can cause a misfire.
If you unplug the injector on cylinder one and nothing changes could mean there's an issue with the fuel injector but could mean there are several other issues too.
I would usually start with the easiest checks first. Check cylinder 1 coil for spark. Carefully place a screwdriver into the coil boot and have someone crank the engine over to check for spark. You'll need someone to help you with this and place the screw driver close to a metal part on the engine to watch for spark. If there isn't any spark you'll have to check the coil circuit with a test light or volt meter to make sure the coil is getting what is needs to function.
You could move cylinder 1 coil to cylinder 2 and also move cylinder 1 spark plug to cylinder 3 to see if the misfire moves to another cylinder. If it doesn't then that means there's either a wiring issue or a engine mechanical failure in that one cylinder.
If you have spark you'll have to check for compression in cylinder one. Most big franchise auto parts suppliers will loan you the compression gauge tool for free. You should have compression in the 120-130psi or higher range. If cylinder 1 is lower than the other cylinders then there is a mechanical problem with the engine valves or piston rings.

If compression is good. The next steps would be to check the fuel injector or wiring if everything else checks out.
I have seen that Hondas have a history of ignition coil failures.

Hope this helps and let us know the outcome of what you find.

There are many things that each cylinder needs to function properly.
You need spark, spark timing, Proper amount of fuel, good compression, enough air getting inside the engine and good timing.
What I was trying direct you to was to find out what it's not by moving parts around to see if there was a change or if nothing happened at all or in other words process of elimination.

Here are a couple of videos to watch to about misfire diagnosis. They use fairly basic tools to do this.

https://youtu.be/KevJNoaywHc?si=zI_w5YifFLtgKPVN

https://youtu.be/UJap80YG_Yc?si=vYIFeL7CJ8XgmnFj

Let us know the what you find.

Yep, the first thing I would do is remove the spark plugs and do a compression test, this guide can help us, that way we know if the engine is in good shape or not.

https://www.2carpros.com/articles/how-to-test-engine-compression

Please go over this guide and get back to us.