Car engine is not starting

I have Toyota 5E- FE engine. My wiring was sort and ECU was was damaged. after i changed both and temporary test from only on wires not from key. then engine was started when i first test, but when i completely setup full wiring and ECU and when i start from key then engine is not come on start position. And i found there is no injector pulse and spark ignition. i don't know what happened. first time all was okay, engine was running very perfectly. but after complete setup of wiring i don't know what happened. i am so sad. I have checking my full wiring and ECU.There is no problem in wiring but there is no injector pulse and ignition spark and engine is not coming in start position. so what i should do? Please help me.