If the vehicle is due for a tune up, it won't hurt to do it. However, 99% of the time, an engine misfire due to a coil, plug, wire. Will set the check engine light which will indicate which cylinder is misfiring. I guess what I am saying is that I can't say for sure that is going to take care of the problem.
Have you checked fuel pump pressure? Have you checked for engine vacuum leaks?
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Tuesday, December 17th, 2013 AT 8:51 AM